Bessel Beam Plane Illumination Microscopy (BBPIM) is a newly developed optical microscopy technique which illuminates the sample with a Bessel beam. The Bessel beam is a non-diffracting beam of light that can be focused to a very small spot. This small spot size allows for high-resolution imaging of small structures. BBPIM has been used to image a variety of biological samples, including cells, bacteria, and viruses.
BBPIM has several advantages over other optical microscopy techniques. First, the Bessel beam is non-diffracting, which means that it does not spread out as it propagates through the sample. This allows for high-resolution imaging of deep structures within the sample. Second, the Bessel beam is very uniform, which means that it provides even illumination of the sample. This results in images with high contrast and clarity.
BBPIM is a powerful tool for imaging biological samples. It offers high resolution, high contrast, and deep penetration. This makes it an ideal technique for studying the structure and function of cells and other biological structures.